4E1P
Crystal structure of the dimerization domain of Lsr2 from Mycobacterium tuberculosis in the P 1 21 1 space group
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 2011-09-16 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54179 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 32.510, 27.030, 56.830 |
| Unit cell angles | 90.00, 94.31, 90.00 |
Refinement procedure
| Resolution | 29.097 - 1.728 |
| R-factor | 0.1613 |
| Rwork | 0.160 |
| R-free | 0.18520 |
| Structure solution method | AB INITIO PHASING |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.914 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | Arcimboldo |
| Refinement software | PHENIX (1.7.2_869) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.400 | 1.820 |
| High resolution limit [Å] | 1.728 | 1.728 |
| Rmerge | 0.092 | 0.308 |
| Number of reflections | 10538 | |
| <I/σ(I)> | 17.1 | 6.9 |
| Completeness [%] | 99.4 | 96.3 |
| Redundancy | 9.8 | 9.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | 26% PEG400, 0.12 M ammonium sulfate, 0.1 M Tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
