4E0W
Crystal structure of the kainate receptor GluK3 ligand binding domain in complex with kainate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-07-26 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.8726 |
| Spacegroup name | P 41 2 2 |
| Unit cell lengths | 68.558, 68.558, 126.883 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.478 - 2.350 |
| R-factor | 0.1816 |
| Rwork | 0.179 |
| R-free | 0.23220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3s9e |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.062 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.16) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.7.3_928) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.478 | 2.480 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.393 | |
| Number of reflections | 13274 | |
| <I/σ(I)> | 9.7 | 2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.9 | 8.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.2 | 293 | 1.8 M SODIUM/POTASSIUM PHOSPHATE, CRYSTALS GROWN IN PRESENCE OF GLUTAMATE SOAKED WITH KAINATE, pH 8.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
