4E0V
Structure of L-amino acid oxidase from the B. jararacussu venom
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE W01B-MX2 |
| Synchrotron site | LNLS |
| Beamline | W01B-MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.458 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 66.385, 72.190, 101.527 |
| Unit cell angles | 90.00, 90.90, 90.00 |
Refinement procedure
| Resolution | 29.020 - 3.100 |
| R-factor | 0.18541 |
| Rwork | 0.181 |
| R-free | 0.25931 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.667 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.020 | 3.210 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.311 | |
| Number of reflections | 16155 | |
| <I/σ(I)> | 2.6 | |
| Completeness [%] | 91.3 | 79.6 |
| Redundancy | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 291 | 0.1 M sodium acetate trihydrate pH 4.6 and 25% (w/v) polyethylene glycol 1000, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
