4E0M
SVQIVYK segment from human Tau (305-311) displayed on 54-membered macrocycle scaffold (form I)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-04-26 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.91609 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 58.320, 25.340, 50.780 |
Unit cell angles | 90.00, 122.19, 90.00 |
Refinement procedure
Resolution | 18.220 - 1.750 |
R-factor | 0.1889 |
Rwork | 0.185 |
R-free | 0.22130 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.290 |
Data scaling software | XSCALE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 18.220 | 1.800 | |
High resolution limit [Å] | 1.750 | 7.830 | 1.750 |
Rmerge | 0.086 | 0.044 | 0.424 |
<I/σ(I)> | 7.83 | 14.97 | 2.5 |
Completeness [%] | 99.0 | 89.4 | 98.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 290 | 0.1M Na/K phosphate buffer pH 6.2, 35% (v/v) 2-methyl-2,4-pentanediol, vapor diffusion, hanging drop, temperature 290K |