4DZR
The crystal structure of protein-(glutamine-N5) methyltransferase (release factor-specific) from Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-02-16 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97931, 0.97948 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 61.766, 61.766, 122.379 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.883 - 2.551 |
R-factor | 0.2097 |
Rwork | 0.207 |
R-free | 0.26890 |
Structure solution method | MAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.204 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | SHELXD |
Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 31.000 | 2.590 |
High resolution limit [Å] | 2.550 | 2.550 |
Rmerge | 0.071 | 0.692 |
Number of reflections | 8235 | |
<I/σ(I)> | 28.8 | 2.2 |
Completeness [%] | 99.4 | 100 |
Redundancy | 4.5 | 4.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289 | 0.2M calcium acetate hydrate, pH 7.5, 20% (w/v) PEG 3350, 0.0125mg/ml Trypsin, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |