4DZN
A de novo designed Coiled Coil CC-pIL
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-10-10 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.7 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 24.723, 40.943, 87.430 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.720 - 1.590 |
| R-factor | 0.15714 |
| Rwork | 0.155 |
| R-free | 0.19844 |
| Structure solution method | AB INITIO PHASING+MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.115 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | ACORN |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.720 | 1.700 |
| High resolution limit [Å] | 1.590 | 1.600 |
| Number of reflections | 11991 | |
| Completeness [%] | 96.1 | 95.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 291 | 0.2 M NH4H2PO4, 0.1 M Tris, 50% v/v MPD, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
