4DZM
A de novo designed Coiled Coil CC-Di
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-08-09 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.7 |
| Spacegroup name | P 62 |
| Unit cell lengths | 23.509, 23.509, 189.598 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.910 - 1.940 |
| R-factor | 0.19472 |
| Rwork | 0.192 |
| R-free | 0.24384 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.665 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELXS |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.910 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Completeness [%] | 97.1 | 87.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 291 | 0.2 M NaCl, 25 % w/v PEG 3350, 0.1 M Bis Tris, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
