4DZG
Crystal structure of Aeromonas hydrophila PliG, a periplasmic lysozyme inhibitor of g-type lysozyme
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2011-04-26 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.54 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 76.259, 76.259, 62.443 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 18.317 - 2.020 |
R-factor | 0.1854 |
Rwork | 0.184 |
R-free | 0.20720 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.073 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.060 | 2.130 |
High resolution limit [Å] | 2.020 | 2.020 |
Number of reflections | 13867 | |
<I/σ(I)> | 16.6 | 2.5 |
Completeness [%] | 98.1 | 92.3 |
Redundancy | 8.9 | 5.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.1M sodium cacodylate pH6.5, 1M sodium citrate, VAPOR DIFFUSION, HANGING DROP, temperature 293K |