4DYY
Crystal Structure of the Cu-adduct of Human H-Ferritin variant MIC1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-04-16 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 0.98 |
Spacegroup name | F 4 3 2 |
Unit cell lengths | 179.594, 179.594, 179.594 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 89.800 - 1.900 |
R-factor | 0.21935 |
Rwork | 0.218 |
R-free | 0.24072 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2cei |
RMSD bond length | 0.007 |
RMSD bond angle | 0.886 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.3.15) |
Phasing software | MOLREP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 103.689 | 89.797 | 2.000 |
High resolution limit [Å] | 1.900 | 6.010 | 1.900 |
Rmerge | 0.045 | 0.492 | |
Number of reflections | 20095 | ||
<I/σ(I)> | 22 | 14.2 | 1.4 |
Completeness [%] | 99.8 | 94.6 | 100 |
Redundancy | 27.1 | 23.2 | 20 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 298 | 50 mM Tris, 5 mM calcium chloride, 8% PEG 1900 MME, 700 micro M cupric chloride, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |