4DYU
The crystal structure of DNA starvation/stationary phase protection protein Dps from Yersinia pestis KIM 10
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-02-16 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97932 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 105.414, 105.102, 191.834 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.192 - 2.750 |
| R-factor | 0.184 |
| Rwork | 0.179 |
| R-free | 0.26890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ak9 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.136 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.200 | 2.800 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Rmerge | 0.196 | 0.709 |
| Number of reflections | 56019 | |
| <I/σ(I)> | 11.2 | 2.3 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 4.9 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 289 | 0.2M Lithium Sulfate, 0.1M MES:NaOH, 35% (v/v) MPD, pH 6, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
