4DYU
The crystal structure of DNA starvation/stationary phase protection protein Dps from Yersinia pestis KIM 10
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-02-16 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97932 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 105.414, 105.102, 191.834 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.192 - 2.750 |
R-factor | 0.184 |
Rwork | 0.179 |
R-free | 0.26890 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ak9 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.136 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | MOLREP |
Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.200 | 2.800 |
High resolution limit [Å] | 2.750 | 2.750 |
Rmerge | 0.196 | 0.709 |
Number of reflections | 56019 | |
<I/σ(I)> | 11.2 | 2.3 |
Completeness [%] | 99.8 | 100 |
Redundancy | 4.9 | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 289 | 0.2M Lithium Sulfate, 0.1M MES:NaOH, 35% (v/v) MPD, pH 6, VAPOR DIFFUSION, SITTING DROP, temperature 289K |