4DXM

Crystal Structure of an ancestral GFP-like protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2010-12-06
Detector	NOIR-1
Wavelength(s)	0.9998
Spacegroup name	C 1 2 1
Unit cell lengths	106.280, 92.710, 89.060
Unit cell angles	90.00, 93.18, 90.00

Refinement procedure

Resolution	29.670 - 1.400
R-factor	0.14485
Rwork	0.143
R-free	0.17917
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.492
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.5.0110)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.670	1.480
High resolution limit [Å]	1.400	1.400
Rmerge		0.378
Number of reflections	169035
<I/σ(I)>	6.1
Completeness [%]	99.9	100
Redundancy	3.5	0.35

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.7	298	0.2M tris, 0.12M magnesium sulfate, 20% PEG 4000, pH 7.7, VAPOR DIFFUSION, HANGING DROP, temperature 198K, temperature 298K