4DX9
ICAP1 in complex with integrin beta 1 cytoplasmic tail
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-12-06 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97860 |
| Spacegroup name | P 1 |
| Unit cell lengths | 75.622, 122.207, 135.269 |
| Unit cell angles | 89.97, 89.99, 108.11 |
Refinement procedure
| Resolution | 50.010 - 2.990 |
| R-factor | 0.2546 |
| Rwork | 0.251 |
| R-free | 0.31194 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.813 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AutoSol |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.010 | 3.110 |
| High resolution limit [Å] | 2.990 | 2.990 |
| Number of reflections | 91718 | |
| <I/σ(I)> | 16.6 | 2.3 |
| Completeness [%] | 98.8 | 98.8 |
| Redundancy | 13.3 | 13.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 1.2M sodium citrate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
