4DWM
Crystal structure of the complex of type I Ribosome inactivating protein with N-acetylglucosamine at 1.62 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 2011-10-04 |
Detector | MARRESEARCH |
Wavelength(s) | 0.97 |
Spacegroup name | H 3 |
Unit cell lengths | 130.198, 130.198, 40.510 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.000 - 1.620 |
R-factor | 0.15808 |
Rwork | 0.156 |
R-free | 0.19283 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3mrw |
RMSD bond length | 0.022 |
RMSD bond angle | 1.873 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 65.100 | 1.650 |
High resolution limit [Å] | 1.620 | 1.620 |
Rmerge | 0.036 | 0.462 |
Number of reflections | 40774 | |
<I/σ(I)> | 27.3 | 2.3 |
Completeness [%] | 99.2 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 298 | 14% PEG 6000, 100mM citrate buffer, pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K |