4DWF

Crystal structure of a HLA-B associated transcript 3 (BAT3) from Homo sapiens at 1.80 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2011-12-01
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.91162, 0.97949, 0.97903
Spacegroup name	P 21 21 2
Unit cell lengths	61.320, 74.090, 43.119
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.330 - 1.800
R-factor	0.1854
Rwork	0.182
R-free	0.24060
Structure solution method	MAD
RMSD bond length	0.017
RMSD bond angle	1.736
Data reduction software	XDS
Data scaling software	XSCALE (December 6, 2010)
Phasing software	SHELX
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	28.330		1.860
High resolution limit [Å]	1.800	3.870	1.800
Rmerge	0.042	0.019	0.487
Number of reflections	18720	3438	3100
<I/σ(I)>	10.74	30.7	1.6
Completeness [%]	97.9	96.2	93.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	277	2.0M (NH4)2SO4, 0.2M K/Na Tartrate, 0.1M Citrate pH 5.6, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K