4DWC
Crystal structure of the glycoprotein Erns from the pestivirus BVDV-1 in complex with Zn ions
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2009-06-20 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.28170 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 106.597, 106.597, 213.007 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 47.660 - 2.890 |
R-factor | 0.1971 |
Rwork | 0.195 |
R-free | 0.22730 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4dvk |
RMSD bond length | 0.010 |
RMSD bond angle | 1.250 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | BUSTER-TNT (BUSTER 2.9.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.660 | 3.050 |
High resolution limit [Å] | 2.890 | 2.890 |
Number of reflections | 16506 | |
Completeness [%] | 98.5 | 90.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 4.6 | 298 | 33% PEG2000 MME, 100mM Na-Acetate, 140mM (NH4)2SO4, 50mM KH2PO4, 50mM ZnCl2, pH 4.6, VAPOR DIFFUSION, temperature 298K |