4DVV
Crystal structure of clade A/E 93TH057 HIV-1 gp120 core in complex with AS-I-261
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2011-06-23 |
Detector | MAR scanner 300 mm plate |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 64.779, 68.079, 94.350 |
Unit cell angles | 90.00, 91.42, 90.00 |
Refinement procedure
Resolution | 26.390 - 1.940 |
R-factor | 0.1829 |
Rwork | 0.181 |
R-free | 0.21520 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3tgt |
RMSD bond length | 0.004 |
RMSD bond angle | 0.856 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASES |
Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 26.390 | 1.970 |
High resolution limit [Å] | 1.940 | 1.940 |
Rmerge | 0.058 | 0.330 |
Number of reflections | 58230 | |
<I/σ(I)> | 22 | 2.23 |
Completeness [%] | 95.4 | 63.4 |
Redundancy | 4.4 | 2.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 10% PEG 8000, 5% iso-propanol, 0.1M HEPES, VAPOR DIFFUSION, HANGING DROP, temperature 293K, pH 7.5 |