4DVJ
Crystal structure of a putative zinc-dependent alcohol dehydrogenase protein from Rhizobium etli CFN 42
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-02-22 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9788 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 185.312, 42.239, 111.265 |
| Unit cell angles | 90.00, 122.39, 90.00 |
Refinement procedure
| Resolution | 48.660 - 1.990 |
| R-factor | 0.1645 |
| Rwork | 0.162 |
| R-free | 0.20618 |
| RMSD bond length | 0.028 |
| RMSD bond angle | 1.992 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AutoSol |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.060 |
| High resolution limit [Å] | 1.990 | 1.990 |
| Rmerge | 0.090 | 0.260 |
| Number of reflections | 50492 | |
| <I/σ(I)> | 7.4 | 5 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7.1 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.2M Na acetate, 0.1M Tris pH 8.5, 25% PEG 4K, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
