4DVE
Crystal structure at 2.1 A of the S-component for biotin from an ECF-type ABC transporter
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-07-16 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.97935 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 89.825, 57.384, 166.865 |
| Unit cell angles | 90.00, 91.07, 90.00 |
Refinement procedure
| Resolution | 48.360 - 2.090 |
| R-factor | 0.1864 |
| Rwork | 0.185 |
| R-free | 0.20280 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.567 |
| Data scaling software | XSCALE |
| Phasing software | SHARP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 48.360 | 2.220 | |
| High resolution limit [Å] | 2.090 | 6.230 | 2.090 |
| Rmerge | 0.059 | 0.034 | 0.438 |
| Number of reflections | 3727 | 14484 | |
| <I/σ(I)> | 15.3 | 41.66 | 2.51 |
| Completeness [%] | 98.4 | 99.8 | 90.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 278 | 0.05-0.2 mM CaCl2, 45-50% PEG400, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 278K |
