4DV7

Crystal structure of the Thermus thermophilus 30S ribosomal subunit with a 16S rRNA mutation, A915G, bound with streptomycin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2010-11-07
Detector	ADSC QUANTUM 315
Wavelength(s)	0.98
Spacegroup name	P 41 21 2
Unit cell lengths	403.366, 403.366, 173.597
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.924 - 3.294
R-factor	0.1698
Rwork	0.168
R-free	0.21030
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.007
RMSD bond angle	1.022
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHENIX (dev_978)
Refinement software	PHENIX (dev_978)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	35.000	35.000	3.420
High resolution limit [Å]	3.294	7.090	3.294
Rmerge	0.091	0.046	0.875
Number of reflections	210955
<I/σ(I)>	12.6
Completeness [%]	98.8	93	99.9
Redundancy	4.8	4.7	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	HANGING DROP	6.5	277	MPD, pH 6.5, HANGING DROP, temperature 277K