4DV4

Crystal structure of the Thermus thermophilus 30S ribosomal subunit with a 16S rRNA mutation, A914G

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2010-11-07
Detector	ADSC QUANTUM 315
Wavelength(s)	0.98
Spacegroup name	P 41 21 2
Unit cell lengths	402.638, 402.638, 174.747
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.661 - 3.651
R-factor	0.1559
Rwork	0.153
R-free	0.20830
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.013
RMSD bond angle	1.620
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHENIX (dev_978)
Refinement software	PHENIX (dev_978)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	35.000	35.000	3.780
High resolution limit [Å]	3.650	7.840	3.650
Rmerge	0.080	0.037	0.686
Number of reflections	155290
<I/σ(I)>	13.5
Completeness [%]	98.3	92.7	99.5
Redundancy	3.2	3.1	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	HANGING DROP	6.5	277	MPD, pH 6.5, HANGING DROP, temperature 277K