4DV3

Crystal structure of the Thermus thermophilus 30S ribosomal subunit with a 16S rRNA mutation, C912A, bound with streptomycin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2010-04-19
Detector	ADSC QUANTUM 315
Wavelength(s)	0.98
Spacegroup name	P 41 21 2
Unit cell lengths	402.977, 402.977, 172.607
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.752 - 3.547
R-factor	0.1689
Rwork	0.166
R-free	0.22020
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.013
RMSD bond angle	1.598
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHENIX (dev_978)
Refinement software	PHENIX (dev_978)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	3.680
High resolution limit [Å]	3.546	7.610	3.546
Rmerge	0.156	0.094	0.770
Number of reflections	164584
<I/σ(I)>	10.4
Completeness [%]	96.6	93.5	90.9
Redundancy	2.8	2.8	2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	HANGING DROP	6.5	277	MPD, pH 6.5, HANGING DROP, temperature 277K