4DV2
Crystal structure of the Thermus thermophilus 30S ribosomal subunit with a 16S rRNA mutation, C912A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-04-19 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 403.743, 403.743, 173.221 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.771 - 3.646 |
| R-factor | 0.1675 |
| Rwork | 0.165 |
| R-free | 0.22300 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.590 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX (dev_978) |
| Refinement software | PHENIX (dev_978) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 3.780 |
| High resolution limit [Å] | 3.646 | 7.820 | 3.646 |
| Rmerge | 0.120 | 0.084 | 0.759 |
| Number of reflections | 153457 | ||
| <I/σ(I)> | 12.2 | ||
| Completeness [%] | 97.2 | 90.8 | 97.5 |
| Redundancy | 3.1 | 3.1 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | HANGING DROP | 6.5 | 277 | MPD, pH 6.5, HANGING DROP, temperature 277K |
