4DV1

Crystal structure of the Thermus thermophilus 30S ribosomal subunit with a 16S rRNA mutation, U20G, bound with streptomycin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2010-11-07
Detector	ADSC QUANTUM 315
Wavelength(s)	0.98
Spacegroup name	P 41 21 2
Unit cell lengths	403.455, 403.455, 173.407
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.930 - 3.849
R-factor	0.1531
Rwork	0.150
R-free	0.21160
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.012
RMSD bond angle	1.520
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHENIX (dev_978)
Refinement software	PHENIX (dev_978)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	35.000	35.000	3.990
High resolution limit [Å]	3.849	8.260	3.849
Rmerge	0.075	0.032	0.724
Number of reflections	131031
<I/σ(I)>	11.8
Completeness [%]	97.3	91.5	99
Redundancy	3	2.9	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	HANGING DROP	6.5	277	MPD, pH 6.5, HANGING DROP, temperature 277K