4DV0

Crystal structure of the Thermus thermophilus 30S ribosomal subunit with a 16S rRNA mutation, U20G

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2010-11-07
Detector	ADSC QUANTUM 315
Wavelength(s)	0.98
Spacegroup name	P 41 21 2
Unit cell lengths	402.108, 402.108, 173.997
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.823 - 3.853
R-factor	0.1504
Rwork	0.147
R-free	0.20590
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.011
RMSD bond angle	1.428
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHENIX (dev_978)
Refinement software	PHENIX (dev_978)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	35.000	35.000	3.990
High resolution limit [Å]	3.850	8.260	3.850
Rmerge	0.071	0.028	0.817
Number of reflections	130659
<I/σ(I)>	12.2
Completeness [%]	97.6	90.9	99.5
Redundancy	3.2	3.2	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	HANGING DROP	6.5	277	MPD, pH 6.5, HANGING DROP, temperature 277K