4DUR

The X-ray Crystal Structure of Full-Length type II Human Plasminogen

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2011-11-04
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.953692
Spacegroup name	P 32 2 1
Unit cell lengths	144.620, 144.620, 233.670
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	125.250 - 2.450
R-factor	0.191
Rwork	0.190
R-free	0.21550
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1qrz 1krn 5hpg 1los
RMSD bond length	0.007
RMSD bond angle	0.960
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASES
Refinement software	BUSTER (2.10.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	125.250	2.580
High resolution limit [Å]	2.450	2.450
Rmerge	0.175	0.855
Number of reflections	104073
<I/σ(I)>	9.2	2.3
Completeness [%]	99.8	98.9
Redundancy	6.9	5.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	277	0.1M sodium citrate, 0.2M sodium acetate, polyethylene glycol 1000, polyethylene glycol 8000, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K