4DTD
Structure and functional characterization of a Vibrio cholerae toxin from the MARTX/VgrG family.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-12-16 |
Detector | PSI PILATUS 6M |
Spacegroup name | P 43 2 2 |
Unit cell lengths | 128.080, 128.080, 76.520 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.860 - 2.500 |
R-factor | 0.188 |
Rwork | 0.187 |
R-free | 0.21300 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.180 |
Refinement software | BUSTER (2.9.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.100 | 2.630 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.070 | 0.560 |
Number of reflections | 22710 | |
<I/σ(I)> | 27.2 | 5 |
Completeness [%] | 100.0 | 99.5 |
Redundancy | 17.3 | 17 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.3 | 293 | mixing 300 nL of protein at 13mg/mL with 100 nL of 2.17 M AmSO4, 0.1 M Bi-Tris pH 7.0 , VAPOR DIFFUSION, SITTING DROP, temperature 293K |