4DT1
Crystal structure of the Psy3-Csm2 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGHT |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-10-15 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 135.123, 50.433, 76.833 |
| Unit cell angles | 90.00, 103.17, 90.00 |
Refinement procedure
| Resolution | 33.094 - 1.899 |
| R-factor | 0.1943 |
| Rwork | 0.192 |
| R-free | 0.22950 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.990 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | PHENIX ((phenix.refine: 1.6_289)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.899 | 1.899 |
| Number of reflections | 38738 | |
| Completeness [%] | 96.6 | 84.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 285 | 100mM Tris-HCl, 10% Glycerol, 14% ethanol, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 285K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 285 | 100mM Tris-HCl, 10% Glycerol, 14% ethanol, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 285K |
