4DSR
Crystal structure of peroxiredoxin Ahp1 from Saccharomyces cerevisiae in reduced form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-04-20 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9791 |
Spacegroup name | H 3 |
Unit cell lengths | 154.463, 154.463, 90.966 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 44.200 - 2.910 |
R-factor | 0.24051 |
Rwork | 0.239 |
R-free | 0.27423 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1tp9 |
RMSD bond length | 0.013 |
RMSD bond angle | 1.401 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.010 |
High resolution limit [Å] | 2.910 | 2.910 |
Rmerge | 0.131 | 0.369 |
Number of reflections | 16465 | |
<I/σ(I)> | 7.68 | 2.991 |
Completeness [%] | 91.3 | 91.5 |
Redundancy | 4 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289 | 25% polyethylene glycol 3350, 0.2M ammonium sulfate, 0.1M HEPES-NaOH, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |