4DSL
Crystal structure of fragment DNA polymerase I from Bacillus stearothermophilus with duplex DNA and Calcium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 12.3.1 |
| Synchrotron site | ALS |
| Beamline | 12.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-02-18 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 88.340, 93.587, 104.618 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.450 |
| R-factor | 0.22875 |
| Rwork | 0.222 |
| R-free | 0.28568 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB 3HT3 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.829 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASES |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.540 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Number of reflections | 30107 | |
| <I/σ(I)> | 7.2 | 1.9 |
| Completeness [%] | 92.1 | 79 |
| Redundancy | 2.7 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 0.1 M HEPES pH 7.5, 2.0 M Ammonium Sulfate, VAPOR DIFFUSION, SITTING DROP |
