4DS7

Crystal structure of yeast calmodulin bound to the C-terminal fragment of spindle pole body protein Spc110

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2011-04-28
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97153
Spacegroup name	P 21 21 2
Unit cell lengths	114.670, 145.444, 49.837
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.150
R-factor	0.1992
Rwork	0.197
R-free	0.24010
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3hr4
RMSD bond length	0.008
RMSD bond angle	0.984
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.190
High resolution limit [Å]	2.150	5.820	2.150
Rmerge	0.070
Number of reflections	47075
<I/σ(I)>	31.184	80.894	3.876
Completeness [%]	99.9	98.6	99.5
Redundancy	15.9	15.7	11.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	4.5	293	0.1M sodium acetate, 0.7M ammonium sulfate, 0.5M guanidine chloride, 0.001M strontium chloride, 0.00005M copper chloride, pH 4.5, 0.0005M LAURYLDIMETHYLAMINE OXIDE, VAPOR DIFFUSION, temperature 293K