Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-06-30 |
| Detector | SBC-3 |
| Wavelength(s) | 0.97901 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.686, 40.487, 46.486 |
| Unit cell angles | 90.00, 108.95, 90.00 |
Refinement procedure
| Resolution | 19.760 - 2.300 |
| R-factor | 0.187 |
| Rwork | 0.184 |
| R-free | 0.25140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1oiv |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.286 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 4.950 | 2.300 |
| Rmerge | 0.134 | 0.115 | 0.403 |
| Number of reflections | 6885 | ||
| <I/σ(I)> | 7.2 | ||
| Completeness [%] | 95.0 | 97.4 | 81.2 |
| Redundancy | 3.2 | 3.3 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 20% PEG-3350, 0.2M CALCIUM ACETATE, pH 7.2, vapor diffusion, hanging drop |
