4DRV

Cell attachment protein VP8* of a human rotavirus specifically interacts with A-type histo-blood group antigen

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E SUPERBRIGHT
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	2010-11-25
Detector	RIGAKU RAXIS HTC
Spacegroup name	P 1 21 1
Unit cell lengths	43.720, 35.220, 43.990
Unit cell angles	90.00, 95.45, 90.00

Refinement procedure

Resolution	27.440 - 1.560
R-factor	0.152
Rwork	0.150
R-free	0.17800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1kqr
RMSD bond length	0.006
RMSD bond angle	1.037
Refinement software	PHENIX ((phenix.refine: 1.7_650))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.440	1.640
High resolution limit [Å]	1.560	1.560
Number of reflections	19362
Completeness [%]	99.4	96.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		4.5	293	30% PEG 1500 Sodium acetate trihydrate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K