4DRS
Crystal structure of Cryptosporidium parvum pyruvate kinase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-07-04 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.99987 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 129.895, 136.941, 77.233 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.710 - 2.500 |
| R-factor | 0.21523 |
| Rwork | 0.213 |
| R-free | 0.24949 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1pkl |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.915 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.800 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.060 | 0.280 |
| Number of reflections | 47349 | |
| <I/σ(I)> | 16.1 | 5.7 |
| Completeness [%] | 97.0 | 98.5 |
| Redundancy | 4.2 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 277 | 0.4-0.8 M ammonium sulfate, 0.1 M sodium acetate, pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
