4DR7
Crystal structure of the Thermus thermophilus (HB8) 30S ribosomal subunit with codon, crystallographically disordered near-cognate transfer RNA anticodon stem-loop mismatched at the second codon position, and streptomycin bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-06-28 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 402.490, 402.490, 174.790 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.909 - 3.750 |
| R-factor | 0.1507 |
| Rwork | 0.148 |
| R-free | 0.20080 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.627 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX (dev_978) |
| Refinement software | PHENIX (dev_978) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.850 |
| High resolution limit [Å] | 3.750 | 16.770 | 3.750 |
| Rmerge | 0.136 | 0.014 | 0.014 |
| Number of reflections | 146049 | 1750 | 10704 |
| <I/σ(I)> | 15.52 | 59.28 | 1.99 |
| Completeness [%] | 99.9 | 95 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | HANGING DROP | 6.5 | 277 | MPD, pH 6.5, HANGING DROP, temperature 277K |
