4DR6
Crystal structure of the Thermus thermophilus (HB8) 30S ribosomal subunit with codon, near-cognate transfer RNA anticodon stem-loop mismatched at the first codon position and streptomycin bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-06-28 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 402.600, 402.600, 174.900 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.484 - 3.300 |
| R-factor | 0.1554 |
| Rwork | 0.153 |
| R-free | 0.19180 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.946 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX (dev_978) |
| Refinement software | PHENIX (dev_978) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.390 |
| High resolution limit [Å] | 3.300 | 14.760 | 3.300 |
| Rmerge | 0.092 | 0.012 | 0.012 |
| Number of reflections | 213602 | 2567 | 15620 |
| <I/σ(I)> | 18.75 | 54.5 | 2.02 |
| Completeness [%] | 99.9 | 96.5 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | HANGING DROP | 6.5 | 277 | MPD, pH 6.5, HANGING DROP, temperature 277K |
