4DR3

Crystal structure of the Thermus thermophilus (HB8) 30S ribosomal subunit with streptomycin bound

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2011-11-07
Detector	ADSC QUANTUM 315
Wavelength(s)	0.98
Spacegroup name	P 41 21 2
Unit cell lengths	404.041, 404.041, 173.527
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.552 - 3.348
R-factor	0.1695
Rwork	0.168
R-free	0.20630
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.006
RMSD bond angle	0.964
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHENIX (dev_978)
Refinement software	PHENIX (dev_978)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	35.000	35.000	3.470
High resolution limit [Å]	3.348	7.200	3.348
Rmerge	0.062	0.029	0.634
Number of reflections	200934
<I/σ(I)>	14.8
Completeness [%]	98.3	92.2	99
Redundancy	3.3	3.3	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	HANGING DROP	6.5	277	MPD, pH 6.5, HANGING DROP, temperature 277K