4DQZ
Crystal Structure of C-terminal Half of Bacterial Hen1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 200 |
Detector technology | IMAGE PLATE |
Collection date | 2011-10-08 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 0.9787 |
Spacegroup name | P 65 |
Unit cell lengths | 68.503, 68.503, 165.660 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 40.420 - 2.301 |
R-factor | 0.2093 |
Rwork | 0.204 |
R-free | 0.25690 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.376 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine: dev_985)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.300 |
Number of reflections | 19215 |
Completeness [%] | 98.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 15% PEG 400, 0.1M NaCl, 0.1M MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |