4DQ7
Structural Investigation of Bacteriophage Phi6 Lysin (V207F mutant)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Collection date | 2011-01-01 |
Wavelength(s) | 1.075 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 62.120, 68.722, 89.893 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.010 - 1.400 |
R-factor | 0.178 |
Rwork | 0.175 |
R-free | 0.19800 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 1.001 |
Data scaling software | SCALEPACK |
Refinement software | PHENIX (1.7.3_928) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.420 |
High resolution limit [Å] | 1.400 | 3.800 | 1.400 |
Rmerge | 0.052 | 0.025 | |
Number of reflections | 73877 | ||
<I/σ(I)> | 12.4 | ||
Completeness [%] | 96.5 | 94.7 | 94.5 |
Redundancy | 4.3 | 4.4 | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 289 | 1.6 M Na-Acetate, 0.1 M Na-Citrate, pH 6.5, vapor diffusion, hanging drop, temperature 289K |