4DQ5
Structural Investigation of Bacteriophage Phi6 Lysin (WT)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Collection date | 2011-01-01 |
| Wavelength(s) | 1.075 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 62.159, 68.785, 90.045 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.670 - 1.395 |
| R-factor | 0.1657 |
| Rwork | 0.165 |
| R-free | 0.17730 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.017 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | HKL2Map |
| Refinement software | PHENIX (1.7.3_928) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 60.000 | 60.000 | 1.420 |
| High resolution limit [Å] | 1.395 | 3.800 | 1.395 |
| Rmerge | 0.058 | 0.033 | 0.638 |
| Number of reflections | 77074 | ||
| <I/σ(I)> | 10.6 | ||
| Completeness [%] | 99.8 | 97.6 | 100 |
| Redundancy | 4.9 | 4.1 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 289 | 1.6 M Na-Acetate, 0.1 M Na-Citrate, pH 6.5, vapor diffusion, hanging drop, temperature 289K |
