4DPZ
Crystal structure of human HRASLS2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-11-25 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.738, 37.078, 45.423 |
| Unit cell angles | 90.00, 113.38, 90.00 |
Refinement procedure
| Resolution | 20.820 - 1.250 |
| R-factor | 0.15147 |
| Rwork | 0.150 |
| R-free | 0.17884 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4dot |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.657 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.290 |
| High resolution limit [Å] | 1.250 | 1.250 |
| Rmerge | 0.047 | 0.285 |
| Number of reflections | 34298 | |
| <I/σ(I)> | 22 | 3.7 |
| Completeness [%] | 98.0 | 99.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 294 | 1.1 ammonium tartrate dibasic, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
