4DOT
Crystal structure of human HRASLS3.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-03-03 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97949 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 62.446, 62.446, 73.922 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.510 - 1.960 |
R-factor | 0.20386 |
Rwork | 0.203 |
R-free | 0.21634 |
Structure solution method | MIR |
RMSD bond length | 0.016 |
RMSD bond angle | 1.598 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | SHARP |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.070 |
High resolution limit [Å] | 1.950 | 1.950 |
Number of reflections | 8365 | |
Completeness [%] | 68.5 | 5.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 295 | 0.1M BIS-TRIS, 28% PEG MME 2000, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 295K |