4DOO
Crystal structure of Arabidopsis thaliana fatty-acid binding protein At3g63170 (AtFAP1)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-11-01 |
Detector | MARMOSAIC 325 mm CCD |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 56.489, 56.901, 139.608 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 100.000 - 1.900 |
Rwork | 0.200 |
R-free | 0.24830 |
Structure solution method | MAD |
RMSD bond length | 0.005 |
RMSD bond angle | 1.205 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 100.000 |
High resolution limit [Å] | 1.900 |
Number of reflections | 36084 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | 0.1 M sodium TAPS, 19% (w/v) PEG 3350, 0.3 M potassium chloride, 2 mM dithiothreitol, pH 8.5, vapor diffusion, hanging drop, temperature 277K |