4DOG
Structures of Vaccinia Virus Uracil-DNA Glycosylase in New Crystal Forms
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2010-09-01 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | P 64 2 2 |
Unit cell lengths | 85.790, 85.790, 123.460 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.550 - 2.300 |
R-factor | 0.23623 |
Rwork | 0.234 |
R-free | 0.27573 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2owq |
RMSD bond length | 0.007 |
RMSD bond angle | 1.139 |
Data reduction software | d*TREK |
Data scaling software | d*TREK |
Phasing software | PHASER |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.930 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.067 | 0.369 |
Number of reflections | 11573 | |
<I/σ(I)> | 12.8 | 3.7 |
Completeness [%] | 92.8 | 94 |
Redundancy | 3.7 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | 1.5M Ammonium sulfate, 10% Glycerol, 0.1M Hepes, 10mM Nickel sulfate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |