4DO2
Crystal Structure of the Rop protein mutant D30P/A31G at resolution 1.4 resolution.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-12-20 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.817 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 26.669, 38.823, 56.644 |
| Unit cell angles | 90.00, 100.87, 90.00 |
Refinement procedure
| Resolution | 55.640 - 1.401 |
| R-factor | 0.1602 |
| Rwork | 0.159 |
| R-free | 0.18770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rop |
| RMSD bond length | 0.027 |
| RMSD bond angle | 2.405 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASES |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.700 | 1.450 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Number of reflections | 22394 | |
| Completeness [%] | 99.5 | 97.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 291 | 45%(v/v) methanol, 50 mM HEPES pH 6.4 and 100 mM Li2SO4, pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
