4DNW
Crystal structure of UVB-resistance protein UVR8
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-10-10 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 1.07214 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 120.646, 61.517, 103.167 |
Unit cell angles | 90.00, 118.00, 90.00 |
Refinement procedure
Resolution | 32.294 - 1.773 |
R-factor | 0.1726 |
Rwork | 0.171 |
R-free | 0.20120 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4dnu |
RMSD bond length | 0.007 |
RMSD bond angle | 0.965 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.830 |
High resolution limit [Å] | 1.770 | 1.770 |
Number of reflections | 63040 | |
Completeness [%] | 97.4 | 92 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9.2 | 291 | 18%(w/v) PEG 8000, 100mM Tris pH9.2, 200mM Magnesium Chloride, VAPOR DIFFUSION, HANGING DROP, temperature 291K |