4DNV
Crystal structure of the W285F mutant of UVB-resistance protein UVR8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-09-10 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97915 |
| Spacegroup name | P 1 |
| Unit cell lengths | 55.571, 67.214, 93.626 |
| Unit cell angles | 85.06, 81.45, 66.04 |
Refinement procedure
| Resolution | 33.016 - 1.999 |
| R-factor | 0.2084 |
| Rwork | 0.207 |
| R-free | 0.23990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4dnu |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.082 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.070 |
| High resolution limit [Å] | 1.999 | 1.999 |
| Number of reflections | 77456 | |
| Completeness [%] | 93.9 | 94.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | 17%(w/v) PEG 8000, 100mM Tris pH8.5, 200mM Magnesium Chloride, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
