4DNN

Crystal structure of the Quaking Qua1 homodimerization domain

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2011-02-01
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9791358, 0.9184018, 0.979569
Spacegroup name	P 21 21 21
Unit cell lengths	33.983, 36.024, 92.814
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.911 - 2.100
R-factor	0.2179
Rwork	0.216
R-free	0.25150
Structure solution method	MAD
RMSD bond length	0.008
RMSD bond angle	1.102
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.7.2_869))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.650
High resolution limit [Å]	2.100	2.100
Number of reflections	7046
<I/σ(I)>	35.1	5.2
Redundancy	4.3	4.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	295	sodium cacodylate, calcium acetate, PEG 600, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 295K