4DNC
Crystal structure of human MOF in complex with MSL1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-03-20 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.98159 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 64.801, 111.518, 66.751 |
| Unit cell angles | 90.00, 111.05, 90.00 |
Refinement procedure
| Resolution | 40.994 - 2.050 |
| R-factor | 0.2123 |
| Rwork | 0.208 |
| R-free | 0.24630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.064 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 100.000 |
| High resolution limit [Å] | 2.050 |
| Number of reflections | 54362 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | 1.6 M Li2SO4, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
