4DN8

Structure of porcine surfactant protein D neck and carbohydrate recognition domain complexed with mannose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2010-10-25
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 63
Unit cell lengths	66.024, 66.024, 66.018
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	14.763 - 2.200
R-factor	0.2028
Rwork	0.199
R-free	0.23730
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	A complex of pig SPD and glycerol solved by molecular replacement in the same space group using human SPD (pdb code 2GGU B chain)
RMSD bond length	0.002
RMSD bond angle	0.542
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.6.3_473))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	2.280
High resolution limit [Å]	2.200	2.200
Rmerge	0.044	0.418
Number of reflections	8327
<I/σ(I)>	33.9	7.25
Completeness [%]	99.5	99.3
Redundancy	10.8	9.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	290	12.3 mg/ml protein in 0.9 % sodium chloride, 5 mM EDTA, 15 mM calcium chloride, and 20 mM mannose was mixed with an equal volume of 0.1 M bistris (pH =6.5), 20% w/v PEG 3350, 200 mM magesium chloride, and 15% v/v glycerol. Prior to data collection crystals were soaked in a 30% w/v D-mannose solution., VAPOR DIFFUSION, HANGING DROP, temperature 290K