4DMT
Crystal structure of a VWF binding collagen III derived triple helical peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-03-02 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.975 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 31.330, 24.430, 39.320 |
| Unit cell angles | 90.00, 103.11, 90.00 |
Refinement procedure
| Resolution | 38.290 - 1.390 |
| R-factor | 0.10847 |
| Rwork | 0.107 |
| R-free | 0.14466 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1V7H poly-ala |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.940 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.290 | 1.470 |
| High resolution limit [Å] | 1.390 | 1.390 |
| Rmerge | 0.107 | 0.572 |
| Number of reflections | 11914 | |
| <I/σ(I)> | 13.8 | 3.8 |
| Completeness [%] | 99.8 | 99.6 |
| Redundancy | 6.9 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.95-1.0 M ammonium tartrate , VAPOR DIFFUSION, HANGING DROP, temperature 291K |
